آدرینا زیست گستر | کروماتوگرافی – آزمایشگاهی – مواد شیمیایی
MECHANISM OF ION EXCHANGE BONDING
Compounds are retained on the sorbent through ionic bonds. Therefore, it is essential that the sorbent and the analyte to be extracted are charged. Generally, the number of molecules with charged cationic groups increases at pH values below the molecules pKa value. The number of molecules with charged anionic groups decreases at pH values below the molecule’s pKa value. To ensure 99% or more ionization, the pH should be at least two pH units below the pKa of the cation and two pH units above the pKa of the anion. Elution occurs by using a solvent to raise the pH above the pKa of the cationic group or to lower the pH below the pKa of the anion to disrupt retention. At this point, the sorbent or compound is neutralized.
These sorbents are composed of a silica backbone bonded with carbon chains terminated by a negatively or positively charged functional group. Ion exchange interactions occur between a sorbent that carries a charge and a compound of opposite charge.
This electrostatic interaction is reversible by neutralizing the sorbent and/or analyte. Ion exchange bonds can also be disrupted by the introduction of a counter ion to compete with the analyte for binding sites on the sorbent.
ANION EXCHANGE SORBENTS & STRUCTURES
Sorbent Structure pKa
Aminopropyl (primary amine) -Si-(CH2)3NH3+ 9.8
N-2-Aminoethyl (primary & secondary amine) -Si-(CH2)3NH2+(CH2)2NH3+ 10.1, 10.9
Diethylamino (tertiary amine) -Si-(CH2)3NH+(CH2CH3)2 10.6
Quaternary Amine Chloride -Si-(CH2)3N+(CH3)3 Cl– Always charged
Quaternary Amine Hydroxide -Si-(CH2)3N+(CH3)3 OH– Always charged
Quaternary Amine Acetate -Si-(CH2)3N+(CH3)3 CH3COO– Always charged
Quaternary Amine Formate -Si-(CH2)3N+(CH3)3 HCOO– Always charged
Polyimine -Si-(CH2)3NH(CH2COOH)(CH2)N(CH2COOH)2
| Units | Description | Part Number |
|---|---|---|
| 30 | Clean-Up Polyimine 1000mg 6mL | CUPAX1M6 |
| 100 | Clean-Up Polyimine 100mg 1mL | CUPAX111 |
| 50 | Clean-Up Polyimine 200mg 3mL | CUPAX123 |
| 1 | Clean-Up Polyimine 48 Well Plate 300mg | WIMPAX13 |
| 50 | Clean-Up Polyimine 500mg 3mL | CUPAX153 |
| 50 | Clean-Up Polyimine 500mg 6mL | CUPAX156 |
| 1 | Clean-Up Polyimine 96 Well Plate 100mg | WSHPAX11 |
| 1 | Clean-Up Polyimine 96 Well Plate 200mg | WSHPAX12 |
| 1 | Clean-Up Polyimine 96 Well Plate 300mg | WSHPAX13 |
