DMEM/F-12

Gibco™ Advanced DMEM/F-12, 500 mL

Catalog number: 12634010

Advanced DMEM/F-12 (Dulbecco’s Modified Eagle Medium/Ham’s F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat.

Features

• With Glucose, Non-Essential Amino Acids, Sodium pyruvate & Phenol Red.
• Without L-glutamine & HEPES.
• Gibco® Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX® II lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Using Advanced DMEM/F-12

• Advanced DMEM/F-12 requires supplementation with 1-5% Fetal Bovine Serum and 4mM L-glutamine or GlutaMAX™ supplement.
• The FBS concentration must be optimized for each cell line to obtain maximum serum reduction.
• Many cell lines do not require adaptation to this media.
• Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.